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Image Search Results
Journal: bioRxiv
Article Title: SUFI: An automated approach to spectral unmixing of fluorescent multiplex images captured in mouse and postmortem human brain tissues
doi: 10.1101/2021.01.28.428639
Figure Lengend Snippet: Experimental workflow, imaging protocols and data analysis pipelines are similar for mouse and human tissues, but they include optimized conditions for sample preparation (see Methods). Multispectral imaging is performed using a Zeiss LSM780 confocal microscope to acquire lambda stacks. We show a single z-plane across the electromagnetic spectrum pseudo colored by wavelength. Spectral signatures are extracted in Matlab using the Vertex Component Analysis. Finally, spectral unmixing is performed using different algorithms to disentangle signals for individual fluorophores.
Article Snippet: Lambda stacks were acquired in z -series using a
Techniques: Imaging, Sample Prep, Microscopy